High Purity Plasmid Miniprep Kit Spin Column DNA Purification Kit

详情

High Purity Plasmid Miniprep Kit 

For research use only

Kit Content

Description

High Purity Plasmid Miniprep Kit is designed for rapid and cost-effective small-scale preparation of high quality plasmid DNA from recombinant E.coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each spin column can recover up to *0 μg of plasmid DNA. The kit can be successfully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and optimal culture volume depend on the plasmid copy number and medium used for cultivation.

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A**0/A**0 ratios between 1.7 and 1.9, and is suitable for applications such as:

Restriction digestion

 PCR

DNA Sequencing

 in vitro transcription

Feature.

Fast - procedure takes only *0 min.

Efficient – can get ****0µg high copy plasmid DNA in one prep.

High purity - Purified DNA is ready for downstream application such as PCR, restriction    digestion.

Store

store RNase A at **0°C, other reagents can be store at room temperature for up to 1 year. Any precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at *7°C before use. After addition of RNase A, the Resuspension Solution can be used for 6 months when stored at 4°C.