M-MLV Reverse Transcription Kit

详情

Contents

Description
The Dongsheng M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. Dongsheng M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Applications
Generation of first strand cDNA for use in:
-PCR, see Protocol for First-strand cDNA Synthesis;
-real-time PCR;
-second strand cDNA synthesis;
DNA labeling;
Analysis of RNA by primer extension.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.

Functionally tested in first strand cDNA synthesis.

Store Buffer
*0m M Tris-HCl (pH 7.5)
**0 mM NaCl
0.1 mM EDTA
1 mM DTT
0.*1%  NP**0
*0%  glycerol

5xFirst-Strand Buffer
**0 mM Tris-HCl (pH 8.3 at *5°C)
**5 mM KCl
*5 mM MgCl2
*0 mM DTT