详情
Rapid Human Haemoglobin Blood（HHB) Test
Cassette
Intended
Use：
HHB test is a rapid, convenient and odorless qualitative,
sandwich dye conjugate immunoassay for the determination of human
haemoglobin (hHb), and is a useful aid in the diagnosis and therapy
of human blood..
Summary And Explanation：
The HHB method was developed by Van Deen in ***4 to detect
occult blood. Boas began to use this method in ***1 to diagnose
gastric bleeding. Since that time, numerous improvements have been
introduced which utilize the peroxides activity of heme. However,
in order to get accurate test results, Several authors have also
found that some criminal
scence with blood test needed, because of the lack of
sensitivity of methods.
Subsequent development of latex immunochemical agglutination
and of the single radial immunodiffusion(SRID) and of the
reverse-passive hemagglutination(RDHAA)have produced test methods
more sensitive to low concentrations of human haemoglobin in
scence. The results of clinical studies indicate that test results
are positive in only about few blood feces. Therefore, a more
sensitive means for detecting human haemoglobin is
important for the diagnosis of human or animal blood that
result can help police know which one is human blood,which one
is animal feces..
HHB test actually detects lower levels of human haemoglobin
than the standard guaiac tests by employing an immunospecific,
double-sandwich capture method without any restriction on
foods and drugs.
Principle：
HHB test is a qualitative, sandwich dye conjugate immunoassay
and employs a unique combination of monoclonal and polyclonal
antibodies to selectively identify hemoglobin in test samples
with a high degree of sensitivity. In less than five minutes,
elevated levels of human hemoglobin as low as 0.2ugHb/ml can
be detected and positive results for high
levels of hemoglobin can be seen in the test as early as one
or two minutes.
As the test sample flows up through the absorbent device, the
labeled antibody-dye conjugate binds to the hemoglobin in the
specimen forming an antibody-antigen complex.
This complex binds to anti hemoglobin antibody in the positive
test reaction zone and produces a pink-rose color band. In the
absence of hemoglobin, there is no line in the positive test
reaction zone. The pink-rose color bands in the control reaction
zone demonstrate that the reagents and devices are functioning
correctly.
Reagents:
1. HHB test one test per foil pouch.
Ingredients: contains a combination of mouse monoclonal
antibodies and polyclonal antibodies (sheep or goat) directed
against human hemoglobin.Mouse monoclonal antibody on a
colloidal gold particle.
2. Buffer solution.
2 ml preservative buffer with 0.5% (NaN3)
Materials Provided:
Each HHB test (one cassette or one strip) individually sealed
in a foil pouch, and sample collection tube
A. Each pouch contains:
1. One immunological human hemoglobin blood test.
2. Desiccant
B.Sample collection tube
Contains: 2 ml preservative buffer with 0.5% (NaN3)
Material required but not supplied:
Timer, sample container and disposable gloves.
No other equipment or reagents are needed.
Storage
Sample collection tube after collecting sample should be stored
refrigerated(**8℃) if not used immediately. Store test device at
***0℃(room temperature). The test is stable until the expiration
date imprinted on the pouch.
Performance Characteristics：
1. SENSITIVITY
Positive results can be seen in the tests when the levels of human
hemoglobin in stain as low as 0.2 ugHb/ml.
2. Specificity:
HHB is specific for human hemoglobin, sample containing following
substances have no effect on the test result.
Turkey
hemoglobin
**0ug/ml
Pig(Pork)hemoglobin
**0ug/ml
Bovine(Cow)hemoglobin **0ug/ml
Goat
hemoglobin
**0ug/ml
Horse
hemoglobin
**0ug/ml
Pigeon
hemoglobin
**0ug/ml
Radish
Peroxidase
***0ug/ml
Quality Control
Built in Quality Control Features
When the test is complete, a pink-purple colored band will show in
the ”C” control area of the test strip on both negative
sample and positive sample. The appearance of the “C” control
band indicates that the test strip is performing properly and
serves as a built in quality control.
More information, please feel free to contact
us.
