详情
The Ultrasensitive C-reactive Protein Test Kit (Immunoturbidimetric
Latex Assay), HsCRP is a quantitative turbidimetric test for the
measurement of CRP in human serum or plasma. 【Reagent composition
】
Diluent（R1） ：PBS buffer *0 mmol/L, PEG ***0, Surfactant.
Antibody（R2）：PBS buffer *0 mmol/L, anti-human CRP sensitized
emulsion, Preservative.
【Storage and Shelf Life】
1. Sored tightly closed at **8ºC .
2. Non-open kit stored tightly closed at **8ºC has *2 month
shelf life, once opening, the kit has one more month at **8ºC
storage
【Applied analyzer】
Spectrophotometer or biochemical analyzer with **0nm
wavelength.
【Sample】
Fresh serum or plasma. Do not use highly hemolized or lipemic
samples. If not determine in time, store at **0 ℃. Avoid repeat
freezing and melting.
【Procedure】
1. Assay method：two point，wavelength **0nm
2. Application on chemistry analyzer:
Sample Reagent（R1/R2） Temperature Reaction
time（T1/T2）
3μL **0μL/*0μL *7℃ **0s/**0s
Unit Reaction Direction Standard curve simulation
formula
mg/L Positive Multi point calibration, non-linearity
calibration mode
3. Determination: Add 3uL samples into **0μL R1, mix thoroughly and
incubate the cuvette at *7℃ for **0 seconds. Mix *0μL R2 and read
the absorbance A1 against the blank. thoroughly and incubate the
cuvette for **0 seconds, read the absorbance A2. The absorbance of
complex is △A（A*-A1）.
Note: different chemistry analyzer has own specifications, but
should be subject to the basic specifications h
