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【Principle of the method】
Anti-human C3 antibodies when mixed with samples containing C3, form insoluble complexes. These complexes cause an absorbance change, dependent upon the C3 concentration of the patient sample, that can be quantified by comparison from a calibrator of know C3 concentration.
【Reagent composition】
Diluent（R1） ：PBS buffer *0 mmol/L, PEG ***0,Surfactant.
Antibody（R2）：PBS buffer *0 mmol/L, Goat serum, anti-human C3,Preservative.
【Storage and Shelf Life】
1.Sored tightly closed at **8ºC.
2.Non-open kit stored tightly closed at  **8ºC has *2 month shelf  life, once opening, the kit has one more month at**8ºC storage。
【Applied analyzer】
Spectrophotometer or biochemical analyzer with **0nm wavelength.
【Sample】
Fresh serum or plasma. Do not use highly hemolized or lipemic samples.If not determine in time, store at**0℃. Avoid repeat freezing and melting.
【Procedure】
 

1. Assay method：two point，wavelength**0nm