The kit uses a
double-antibody sandwich enzyme-linked immunosorbent assay (ELISA)
to assay the level of Human Folic acid(FA)in samples. Add Folic
acid(FA)to monoclonal antibody Enzyme well which is pre-coated with
Human Folic acid(FA)monoclonal antibody, incubation; then, add
Folic acid(FA) antibodies labeled with biotin, and combined with
Streptavidin-HRP to form immune complex; then carry out incubation
and washing again to remove the uncombined enzyme. Then add
Chromogen Solution A, B, the color of the liquid changes into the
blue, And at the effect of acid, the color finally becomes yellow.
The chroma of color and the concenthumanion of the Human Substance
Folic acid(FA)of sample were positively
correlated.
