Prime Taq DNA Polymerase.

详情

* Description

Prime Taq DNA Polymerase is a  high quality recombinant enzyme and catalyze 5\' →3\'  exonuclease activity. It is provided with 10X reaction buffer  that contains PCR enhancers. This reaction buffer will enable or improve sub-optimal PCR caused by templates that have a high degree of secondary structure or that are GC-rich.


* Model No.

Cat. No. G-1000▶ 250 Units X 1 - with 10 mM dNTPs Mixture
Cat. No. G-1001▶ 250 Units X 2 - with 10 mM dNTPs Mixture
Cat. No. G-1002▶ 250 Units X 4 - with 10 mM dNTPs Mixture

Cat. No. G-1000-1▶ 250 Units X 1 - without 10 mM dNTPs Mixture
Cat. No. G-1001-1▶ 250 Units X 2 - without 10 mM dNTPs Mixture
Cat. No. G-1002-1▶ 250 Units X 4 - without 10 mM dNTPs Mixture
 

* Specifications

▶ Prime Taq DNA Polymerase: 5 units/μl

▶ Storage buffer: 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.5 mM EDTA, 0.1 mM DTT, 0.5% Tween20, 0.5% Nonidet P-40 and 50% Glycerol

▶ 10X reaction buffer: Tris-HCl (pH 9.0), PCR enhancers, (NH4)2SO4
 and 20 mM MgCl2

▶ 10 mM dNTPs Mixture: 2.5 mM each of dATP, dCTP, dGTP and dTTP

* Product Features

▶ General PCR enzyme
▶ Guarantees reproducible test result by its characteristic of high purity
▶ Amplifies target DNA by optimized buffer system
▶ Easily obtains under 5 Kb of DNA amplified products
▶ Prime Taq DNA Polymerase can be used in T-vector cloning

* Buffers and Reagents.
▶ Storage buffer : 20mM Tris -HCI(pH 8.0), 100mM KCI 0.5mM EDTA, 0.1 mM DTT, 0.5% Tween 20, 0, 5% Nonident P-40 and 50% Glycetol
▶ 10mM dNTPs mixture :
2, 5 mM each of dATP, dCTP, dGTP, and dTTP