Methyltetrazine-activated biotinylation reagent that reacts with
TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder
reaction to form a stable covalent bond and does not require
Cu-catalyst or elevated temperatures. The inverse-electron demand
Diels-Alder cycloaddition reaction of TCO with tetrazines is a
bioorthogonal reaction that possesses exceptional kinetics (k >
**0 *1 s*1) and selectivity. Such excellent reaction rate constants
are unparalleled by any other bioorthogonal reaction pair described
to date.
