Biotin-PEG*-Maleimide enables simple and efficient biotinylation of
antibodies, cysteine-containing peptides and other thiol-containing
molecules. The maleimide group reacts specifically and efficiently
with reduced thiols (sulfhydryl groups, SH) at pH 6.5 to 7.5 to
form stable thioether bonds. he hydrophilic polyethylene glycol
(PEG) spacer arm imparts water solubility that is transferred to
the labeled molecule, thus reducing aggregation of labeled proteins
stored in solution.
