Theoretical activity: *8.1 U/mg
protein (S-rSA: Biotin=1:4 (mol: mol))
Activity:≥*5 U/mg protein (determined by
modified Green method)
Purity:≥*5% (SDS-PAGE detection)
Concentration determination: **0nm
absorption method, molar absorption coefficient 1.*8 × **5 (mol/L)
- 1 • cm*1, that is, c (g/L)=A**0nm/2.*0
Endotoxin:≤5EU/mg
Source: Escherichia coli
Storage condition: -
*0℃
Validity: 2 years
Streptavidin (SA) is a homotetramer
protein secreted by Streptomyces avidinii during its growth. Like
avidin, one mole of streptavidin can bind four moles of biotin,
which has high affinity with biotin. Because SA does not contain
sugar groups, it has a lower non-specific binding level than avidin
in detection and application, and has been widely used in
enzyme-linked immunosorbent assay, immunohistochemistry,
time-resolved immunofluorescence (TRFIA), quantitative PCR,
single-stranded DNA preparation, biological component purification,
monoclonal antibody preparation and other biotechnology
fields.
However, the adsorbability of
streptavidin is poor, and it is difficult to adsorb onto NC
membrane or embed it in practical applications. Sticky recombinant
streptavidin (S-rSA) is mainly used to solve this problem. It not
only has the core sequence related to activity, but also has better
adsorbability, stability, solubility and other aspects than natural
SA, especially on NC membrane.
Scope of
application
In many applications, such as
immunocytochemistry, ELISA and immunoblotting, streptavidin
alkaline phosphatase can be used to detect biotinylated molecules,
such as antibodies.
Immunocytochemistry
For most applications, 1:**0
working dilution in TBS is sufficient. At this dilution, about ***0
tests (**0ul per slide) can be carried out for each package. It is
recommended to use TBS instead of PBS in all diluents and washings
to maintain maximum enzyme activity.
