Hygromycin B, **0 mg
Inhibits protein biosynthesis in pro- and eukaryotes by altering
translocation during ribosomal chain elongation, therefore
resulting in frame reading errors and abortion of chain elongation.
Selection marker for hph transfected cells.
Stock solution: *****0 mg/ml in H2O or PBS. Storage of stock
solution at **0 °C. Working concentration: ******0 µg/ml.
Hygromycin B binds near the A site of *6S rRNA and induces errors
in the code by inhibition of translocation of peptydil-tRNA,
however to a lesser extent compared to other aminoglycosides.
Hygromycin B is efficient against bacteria, fungi and other
eukaryotic cells, but more often used for the selection of
eukaryotic cells including yeasts, such as, P. pastoris and S.
cerevisiae, and mammalian cell lines, such as, CHO.
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