In laboratories, the most common experimental
objects are cells. The safety of cells is related to the accuracy
of experimental results. Therefore, they need to be stored in a
low-temperature environment. At this time, experimenters usually
choose a cell freezing container, using ***6° liquid nitrogen to
maintain low temperature. Its vacuum structure can provide a
stable low-temperature environment for cells. However, there are
also disadvantages. Liquid nitrogen is easily volatile. Although
the vacuum structure can effectively reduce it, the surrounding
environment will also have an impact. Therefore, when
usingcell freezing
containers, there are
requirements for the storage environment. Ambient temperature
requirements: A suitable temperature will reduce the
volatilization
of liquid nitrogen, while an excessively high temperature will
accelerate the volatilization. Just like summer and winter, the temperature
difference between the two seasons is large, and the
volatilization in summer will be much higher than that in
winter. The suitable temperature for cell freezing
containers is about *0°, and they can be stored in a cool room or
dark place, away from direct sunlight and high temperature
environments, to reduce the impact of heat on the
tank. Ventilation requirements: It should be placed in a well-ventilated area,
away from sources of fire and flammable materials. In order to
prevent the discharge of volatile liquid nitrogen, the
environment is too sealed and cannot dissipate quickly. It also
competes with oxygen for space, causing workers to suffer from
hypoxia and suffocation. Storage requirements: The inner and outer bladders of the container
are only connected by the neck tube, which is relatively fragile.
To prevent the neck tube from rupturing due to stress, we should
store it vertically. At the same time, pay attention to avoid
collisions during the movement of the jar to prevent the neck
tube connection from falling off and causing damage to the
vacuum. This is the storage environment requirement of
cell freezing containers.
