Let you distinguish at a glance the factors that cause poor separation of serum separation gel

详情

What is separation glue?
It forms an isolation layer between cell components and serum (plasma), effectively preventing the exchange of substances between blood cells and serum (plasma), and guarantees the stability of serum (plasma) components within a certain period of time. It is a high-molecular polymer substance, insoluble in water, inert, and a viscous liquid with thixotropy. The density is between 1.***1.*5 mmol/L. It has oxidation resistance, high temperature resistance, low temperature resistance, and air tightness. Good to wait for the advantages. The serum density is 1.****1.**1 mmol/L, and the blood cell density is 1.****1.**5. Due to the specific gravity, the separating gel is just between the serum and blood cells, so under normal circumstances, the blood will appear in sequence after centrifugation: serum, separating gel, and blood cells. 3 floors.
Reasons for poor separation effect of separation gel
 
1. Separation glue quality
The specific gravity of the separation gel is between serum (plasma) and blood cells, which is the physical basis for the reversibility of the separation gel and the separation of serum (plasma). If the quality of the separation gel for blood collection tubes is poor and the specific gravity cannot meet the requirements, it will inevitably affect the separation of serum (plasma), and the separation gel and serum (plasma) are likely to be intertwined.
 
2. Incomplete blood clotting
After centrifugation, sometimes the separation of the separation gel compartment and serum, blood clots is incomplete, and fibrin filaments appear in the serum. The reason is often that the blood did not completely coagulate before centrifugation.
 
3. Centrifugation temperature
The centrifugation temperature has a significant effect on the separation of serum by the gel coagulation tube. In the inert separation gel accelerator tube separated by ordinary centrifuge at room temperature, the serum is clear, but oily beads of different sizes appear in *5% to *0% of the samples. However, no oily beads were found in the serum separated from the test tube centrifuged in a low-temperature centrifuge. When the temperature exceeds the storage temperature required by the separation hose, the inert separation gel will dissolve in the serum. It will not only block and contaminate the sample needle and reaction cup of the biochemical analyzer, but also have a relatively large impact on the results of some biochemical assays.
 
4. Centrifugal operation
Centrifugation is an important part of the preparation of high-quality serum samples with the separation gel coagulation tube. Generally, the blood collection tube separation gel requires that the blood is collected by **0° and gently inverted and mixed for 8 to *0 times, and placed in an upright position for *0 minutes after the sample is fully coagulated. Centrifuge for *0 minutes in a centrifuge, and maintain the centrifugal force at ***0～***0×g, so that the serum (plasma) and the blood clot are completely separated by the separating gel. If the centrifugal force is too low, the force acting on the separation gel will be weak, and the separation gel will have poor reversibility. Fibrin condensate may stay in the serum or colloid layer, which can easily block the sample needle of the automatic analyzer. On the contrary, if the centrifugation time is too long, it is difficult to form a network structure after the hydrogen bond is cut in the separation colloidal aggregate, and the thixotropy is lost.
 
5. Serum/plasma density
The density of serum (plasma) is the main reason that affects the floating of the separating gel, not its viscosity. Any factor that causes the density of serum (plasma) to increase and exceeds the density of the separation gel can cause abnormal reversion of the separation gel.