revolutionary transfection reagent By guilin peptide technology limited,

revolutionary transfection reagent

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获取最新报价

2 Gram Minimum Order

国家:

China

型号:

离岸价格:

（面議）获取最新报价

位置:

guilin

最小订单价格:

最小订单:

2 Gram

包装细节:

交货时间:

1 day

供应能力:

100 Gram per Month

付款方式:

T/T

產品組 :

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产品规格

详情

Polycysteine-dendritic poly(His,Arg)1:5

Polycysteine-dendritic poly(His,Arg)1:5(CHR) is a revolutionary transfection reagent that isn't made from cytotoxic PEI. It is biodegradable, no cumulative cytotoxicity, and high transfection efficiency. It has been successful in the large scale production of r-proteins and antibodies.
**6USD/**0mg (equal to **0ml, gene therapy research)
**9USD/2g (r-protein production)

Suitable for
DNA, siRNA, protein, peptide and antibody

Transfection Protocol

Description

CHR (see note A) is a revolutionary transfection reagent that isn't made from cytotoxic PEI. CHR has been developed to cater for many cell lines. Arginine residues play a critical role in intracellular uptake. Histidine has buffering capacity in the acidic environment of endosomes. Disulfide bonds were reported to show significantly increased transfection efficiency and cell viability.

Cell Lines Successfully Transfected Using CHR:

HEK***3, COS*7, C2C*2, HeLa, NIH/3T3, CHO, PC**2, MCF7, U*2 OS, A**9, LNCaP, PC*3, HCT **6, HT**9, Hep G2, Huh*7, K***2, Jurkat, RAW **4.7, H9c2, TF*1, HUVEC, Cardiomyocytes , THP*1, MDA-MB***3, Raji, HepaRG, Ba/F3, U***7, L***9, ***7

Storage Conditions:

Ina dessicator dryer with anhydrous calcium chloride at RT

Recommended Protocol

We recommend using a **-well plate format to optimize transfection conditions for a particular cell type.

Plate cells one day before the transfection experiment so that cells will be approximately *5% confluent on the day of transfection.

Preparation of CHR& pDNA complexes:
CHR solution: Combine *0 mg CHR with *0 ml DMEM (pH 6.5 for CHO, see note B).
Combine 0.5 ug pDNA with *0 uL DMEM.
*0 uL CHR solution was added to this *0 uL pDNA-DMEM gently and incubated for *0 minutes at room temperature.
*0 uL CHR /pDNA complexes were added into each well while gently swirling the plate.
Incubate cell at *7 C in a CO2 incubator. The transfection efficiency of reporter gene could be analysis at ****8 hours after adding the complexes.

Gene transfection in suspension cells:
At 1hour after cell seeding, the CHR/pDNA complexes can be added into cells followed transfection assay at ****8 hours after transfection.

Note A
CHR free base cannotget any transfection, only CHR HCl can transfect cells.

Note B
primary BAE cell line (pH 4.5)
MDA-MB***1 cells (pH 6.2)
primary cell line, NHDF(pH 5.0)
CHO (pH 6.5)
CRL****0 (pH 6.8)

国家:	China
型号:	-
离岸价格:	（面議）获取最新报价
位置:	guilin
最小订单价格:	-
最小订单:	2 Gram
包装细节:	2g
交货时间:	1 day
供应能力:	100 Gram per Month
付款方式:	T/T
產品組 :	ç»�è��è½¬æ��è¯�å��