China
联系人 wei
Zhangjiakou, Hebei
Taq DNA polymerase was isolated and purified from E.coli with a
molecular weight of *4 KD. It has 5 '- 3' DNA polymerase activity
and 5 '- 3' exonuclease activity, but no 3 '- 5' exonuclease
activity. In the PCR reaction, Taq DNA polymerase was extended at a
rate of **2 KB / min, and the product had a 3 'end band, which
could be directly used for t / a vector cloning. The enzyme is
stable and specific without contamination of exogenous nuclease and
bacterial genomic DNA.Activity definition 1 unit (U) Taq DNA
polymerase activity was defined as the amount of enzyme required to
add *0 nmol of deoxynucleotides into acid insoluble substances
using the activated salmon sperm DNA as template at *4 ƒ for *0
minutes.
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Quality Control The purity of Taq DNA polymerase detected by SDS-PAGE was more than *9%, and no exogenous nuclease activity was detected by detection; there was no host residual DNA detected by PCR method; single copy gene in human genome could be amplified effectively; no obvious activity change was found after storage at room temperature for one week. Enzyme storage buffer *0 mM Tris-HCl (pH 8.0);0.1 mMEDTA;1 mMDTT;**0 mMKCl ; Stabilizers; *0% glycerol *0×Taq Buffer **0mm Tris HCl (pH 8.4); **0mm KCl; *5mm MgCl2; other ingredients. Scope of application It is generally used for PCR amplification of DNA fragments, DNA labeling, primer extension, sequencing, and addition of a at the flat end. The product can be directly used for t / a vector cloning. matters needing attention When taking Taq DNA ploymerase for PCR reaction, please use the sterilized tip. |
| 国家: | China |
| 型号: | - |
| 离岸价格: | 30 ~ 40 / ( Negotiable ) 获取最新报价 |
| 位置: | China |
| 最小订单价格: | 30 |
| 最小订单: | 1 Box |
| 包装细节: | box |
| 交货时间: | 15-20days |
| 供应能力: | 1000 Case per Month |
| 付款方式: | D/P, D/A, L/C, T/T, Western Union, PayPal |
| 產品組 : | Taq DNA |
