Thermostable Alpha Amylase

Thermostable Alpha Amylase Highlights

A very robust Alpha-amylase with exceptional thermostability. Ideal for medium-temperature cooking process in fuel ethanol production:

• Enhanced liquefaction efficacy
• Decreased formation of non-fermentable sugars
• Fits to various starch materials, e.g. tapioca, corn, wheat and rice

Thermostable Alpha Amylase Description

Boli HS***0 is a thermostable Alpha-Amylase derived from a modified strain of Bacillus licheniformis. It is an endoamylase that hydrolyzes alpha*1,*-glucosidic bonds to quickly reduce the viscosity of gelatinized starch, producing soluble dextrins and oligosaccharides under various process conditions.

Thermostable Alpha Amylase Characteristics

Thermostable Alpha Amylase Effect Of PH

Boli HS***0 is active over a wide pH range depending on the actual applications. For maximum activity, the optimal pH is 6.0 ~ 6.4. The stable pH range is 5.0 ~ *0.0 at temperature of *5°C. The exact pH optimum depends on actual processing conditions, such as temperature, substrate concentration and time.

Thermostable Alpha Amylase Effect Of Temperature

Optimum temperature of Boli HS***0 for interval liquefaction is above *0°C. It liquefies starch substrates promptly at *5 ~ **0°C. Boli HS***0 demonstrates exceptional thermal stability at temperatures up to **5 ~ **0°C, maintains its stability for 5 ~7 minutes and keeps on liquefying rapidly. The exact temperature optimum of this product depends on the actual processing conditions, including pH, substrate concentration and processing time.

Thermostable Alpha Amylase Concentration Of Calcium

The presence of calcium increases the thermal stability of Boli HS***0. It requires a very low calcium concentration and calcium additions of *0 ~ *0 ppm (dry solid basis) are sufficient to ensure its enzyme stability.

Thermostable Alpha Amylase Inhibitors

Copper, titanium, and cobalt ions are moderate inhibitors for Boli HS***0, whereas aluminum, lead and zinc ions act as strong inhibitors.

Thermostable Alpha Amylase Usage Guiedlines

• Step 1. Adjust pH to 6.0 ~ 6.4
• Step 2. Add calcium (*0 ~ *0 ppm dry solid basis) *
• Step 3. Add Boli HS***0**
• Step 4. Spary the slurry at **5 ~ **0ºC for 5 minutes
• Step 5. Lower the temperature to *0 ~ **0ºC and keep for **0 minutes

Thermostable Alpha Amylase Note:

• Calcium is not needed when extremely hard water (above *0 German degrees) is used.
• Actual dosage of Boli HS***0 depends on the starch substrate, application, process, substrate concentration and other factors. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage is 0.4 ~ 0.6 kg/TDS.