Pfu DNA Polymerase

详情

Description

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is *0 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 0.2~0.4kb/min(*0~*5°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

 Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of *0 nmole of dNTPs into an acid-insoluble form in *0 minutes at *0°C using hering sperm DNA as substrate.

 Storage Buffer

*0mM TrisCl ( pH8.0), **0mM KCl, 3mM MgCl2 1mM DTT,0.1% NP**0 ,0.1% Tween*0, 0.2mg/ml BSA, *0% (v/v) glycerol

 *0X Pfu Buffer with MgSO4 

**0mM Tris-HCl(pH8.8,*5°C), **0mM KCl, **0mM (NH4)2SO4,  *0mM MgSO4, 1.0% Triton®X***0, 1mg/ml BSA

.Applications

• High-fidelity PCR and primer-extension reactions.

• High fidelity PCR for cloning into blunt-ended vectors.

• Site-directed mutagenesis.