Product: 2*Taq PCR Master (without dye)
Cat. No.: T***4
Price: $5/ml (negotiable if bulk purchase)
Storage conditions: **0℃
Product description
The concentration of Taq-ASPCRMix is 2×. It is easy and quick to
use and can reduce contamination during PCR operation. When using,
just take an appropriate amount of 2×Taq-AS PCR Mix, add template
and primers, and add ddH2O to make up the volume, so that the
concentration of the reaction system is 1×, and then you can carry
out PCR reaction. The 3' end of the PCR product has a prominent A
base and can be directly used for T/A cloning after
purification.
The TaqDNA polymerase in this Mix is a screened Taq-AS (Advanced
Strong) DNA Polymerase mutant, which can efficiently amplify DNA
fragments ≤5kb and has good inhibitor tolerance. Its amplification
speed is about 3 times that of ordinary Taq DNA polymerase, so it
can greatly reduce the time required for the PCR extension process,
thereby shortening the entire PCR reaction. Different from the fast
Taq polymerase based on the fusion protein principle, the use of
Taq-AS is closer to WT-Taq, and it is not easy to have
electrophoretic band diffusion, dragging or fragment size
changes.
Quality control
Nuclease activity detection
*5μl 2×Taq-AS PCR Mix and **0ng supercoiled plasmid DNA were
prepared into a *0μl reaction system. After incubation at *7℃ for
4h, less than *0% of the plasmid DNA was converted into a nicked or
linear state by agarose gel electrophoresis.
Nonspecific nuclease activity detection
*5μl 2×Taq-AS PCR Mix and *5ng double-stranded DNA fragment were
prepared into a *0μl reaction system. After incubation at *7℃ for
*6h, agarose gel electrophoresis was used to detect that there was
no change in the double-stranded DNA substrate.
国家: | China |
型号: | - |
离岸价格: | 5 ~ 5 / Milliliter 获取最新报价 |
位置: | - |
最小订单价格: | 5 per Milliliter |
最小订单: | 100 Milliliter |
包装细节: | - |
交货时间: | 7-15 days if in stock |
供应能力: | 1000 Milliliter per Month |
付款方式: | T/T |
產品組 : | - |