详情
miRNA stem-loop fluorescence quantitative kit
Cat.: MR******5T, MR******0T
Price: $**5 for**0T $*8 for *5T $*6 for *0T
(negotiable if bulk purhcase)
Storage conditions: transported on dry ice or blue ice, stored at
**0°C away from light, avoid repeated freezing and thawing, valid
for *2 months. If the amount used each time is small, it is
recommended to use in small portions.
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Components
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*5T
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*0T
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2× miRNA
SYBR Mastermix
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1.0ml ×2
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1.0ml ×4
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Tag Primer
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*0ul
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**0ul
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ddH2O
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1.0ml ×2
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**0μl
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Product Introduction
miRNA Stem-loop SYBR qPCR Kit uses the chemically modified
hot-start polymerase produced by our company and uses the SYBR
Green I chimeric fluorescence method to perform fluorescent
amplification of miRNA. It has high specificity and can
effectively avoid primer dimer formation and other forms of
non-specific amplification. The reaction buffer has been fully
optimized, with high PCR amplification efficiency, strong
fluorescent signal, and higher reaction sensitivity.
Product Features
1. Using chemically modified hot-start polymerase, the reaction
specificity is high, which can effectively avoid non-specific
amplification;
2. The reaction buffer has been fully optimized, the PCR
amplification efficiency is high, and the reaction sensitivity is
better than many similar products.
Quality Control
Using the synthesized U6 as the sample, using this kit, stem-loop
reverse transcription, and qPCR according to the instructions,
the CT value is less than *0.
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