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国家:
China
型号:
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离岸价格:
位置:
China
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最小订单:
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包装细节:
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交货时间:
7-15 days if in stock
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產品組 :
CelGreen nucleic acid
Dye
CAT:CG**1
U.S.$*0.*0
for **0ul (negotiable) Storage:Room temperature and away from light.
Characteristics of CelGreen nucleic
acid dye
● Non-toxic: CelGreen's unique
oiliness and large molecular weight make it unable to penetrate the
cell membrane into the cell, and Ames' test results also show that
the mutagenicity of the dye is far less than EB.
● High sensitivity: suitable for
electrophoretic staining of fragments of various sizes, with less
effect on nucleic acid migration than SYBR Green I.
● High stability: suitable for
using microwave or other heating methods to prepare agarose gel;
Extremely stable in acid or alkali buffers at room temperature,
strong light resistance.
● High signal-to-noise ratio:
sample fluorescence signal is strong, background signal is
low.
● Simple operation: Like EB, the
dye does not degrade in the process of preforming glue and
electrophoresis; The dyeing process after electrophoresis also
takes only *0 minutes without decolorization or rinsing, and can be
directly observed with the ultraviolet gel
transmisometer.
● Wide range of application: you
can choose pre-electrophoresis dyeing (glue dyeing) or
post-electrophoresis dyeing (bubble dyeing); Suitable for agarose
gel or polyacrylamide gel electrophoresis; Can be used for dsDNA,
ssDNA or RNA staining.
● The best excitation can be
obtained near **4nm visible light.
Usage
1. Glue dyeing method (same as EB)
(recommended method)
(1) Add CelGreen nucleic acid dye
when making glue (for example: add 5μL CelGreen *0,**0× storage
solution per *0mL agarose solution, and so on).
(2) Electrophoresis was performed
according to conventional methods.
Note:
The amount of dye used in this
method is relatively small. **0 μL of dye can make about **0 pieces
of *0mL glue.
Because CelGreen has good thermal
stability, it can be added directly in hot agarose solution without
waiting for the solution to cool. Shake, oscillate, or flip to
ensure that the dye is well mixed. Alternatively, the CelGreen
reservoir can be added to the agarose powder and electrophoresis
buffer, and then heated in the microwave or other common method to
prepare the agarose gel. CelGreen is compatible with all commonly
used electrophoresis buffer solutions.
If you always see band dispersion
or separation is not ideal, it is recommended to use bubble dyeing
to confirm whether the problem is related to the dye. If the
problem persists after dyeing, the problem is not related to the
dye, please try: reduce the agarose concentration; Use longer gels;
Extend the gel time to ensure clear edges; Improve the sampling
technique or choose blister dyeing method.
This method is not suitable for
prefabricated polyacrylamide gel, please use the foam dyeing method
for polyacrylamide gel.
2. Blister dyeing
(1) Electrophoresis was performed
according to conventional methods.
(2) The CelGreen *0,**0× storage
solution was diluted about 3,**0 times with H2O into 0.1M NaCl to
make a 3× dyeing solution. (For example, *5μL CelGreen *0,**0×
reservoir and 5mL 1M NaCl are added to *5mL H2O).
(3) Place the gel carefully into a
suitable container, such as a polypropylene container. Slowly add a
sufficient amount of 3× dye solution to immerse the gel. The
optimal dyeing time varies slightly according to the gel thickness
and agarose concentration. For gels containing 3.***0% acrylamide,
the dyeing time is usually between *0min and 1h, and increases with
the increase of acrylamide content.
(4) The results were observed by
**0nm visible light system.
Note:
When dyeing with bubble dyeing
method, the amount of dye is more. A single use of the dye solution
can be reused for about 3 times.
3× CelGreen dyeing solution can be
prepared in large quantities and stored away from light at room
temperature until used up.
3. PAGE steps for nucleic acid
electrophoresis:
(1) Put the gel prepared by TBE
into the electrophoresis tank and hold the edge with a
clamp.
(2) Fill the buffer tank with 5×TBE
in the same batch as the gel solution. Use a syringe to remove air
bubbles from the bottom of the gel.
(3) Use a syringe to draw 1×TBE to
flush the sample addition hole. The DNA sample was mixed with an
appropriate amount of 6× gel loading buffer, and added into the
sampling hole with a micro-pipette.
(4) Connect the electrode to the
power supply (positive terminal slot), turn on the power supply
generally *0V; 1 to 8V/cm. Electrophoresis was performed for
9h.
(5) Electrophoresis until the
standard reference dye migrates to the desired position (generally,
electrophoresis until the xylene is completely removed, and the
bromophenol blue is stopped 2 ~ *5px from the bottom edge). Turn
off the power, unplug and discard the electrophoresis solution from
the bath.
(6) Take the gel off and put it in
the dyeing dish, add 3XCelGreen 1X buffer for oscillating dyeing
for ****0 minutes, and place it in the ultraviolet
detection.
Note:
PAGE glue is different from
aglyceride sugar gel, which can not be pre-dyed or spot-dyed. Can
only use foam dyeing method color, because polyacrylamide is
relatively dense, the dye is not easy to go deep, the color effect
is not as good as Joan ester sugar gel.
Special reminder:
1. If you are using an ultraviolet
imager, please select CelRed; If you use a laser imager or wish to
observe in visible light, choose CelGreen.
2. In rare cases, the DNA sample
after the plasmid is cut by certain enzymes will have a tailing and
resolution reduction, in which case it is recommended to try two
staining methods at the same time to determine which method is more
appropriate.
国家: | China |
型号: | - |
离岸价格: | 获取最新报价 |
位置: | China |
最小订单价格: | - |
最小订单: | - |
包装细节: | - |
交货时间: | 7-15 days if in stock |
供应能力: | - |
付款方式: | T/T, L/C, Western Union, Money Gram, PayPal, Other |
產品組 : | Molecular Biology |