miRNA tailing dye method fluorescence quantitative PCR kit

详情

miRNA tailing dye method fluorescence quantitative PCR kit

Cat. No.: MR***2

Price: $**0/**0T (negotiable if bulk purchase)

Storage conditions: **0℃, valid for 1 year, avoid repeated freezing and thawing

Product introduction

miRNA PolyA SYBR qPCR Kit uses LABLEAD's chemically modified hot-start polymerase and SYBR Green I chimeric fluorescence method for miRNA fluorescence amplification. It has high specificity and can effectively avoid primer dimer formation and other forms of non-specific amplification. The reaction buffer is fully optimized, the PCR amplification efficiency is high, the fluorescence signal is strong, and the reaction sensitivity is better than many similar products. The kit is equipped with an optimized Tag Primer for free, which makes the amplification effect of miRNA more guaranteed.

Product features

The chemically modified hot-start polymerase is used, the reaction specificity is high, and non-specific amplification can be effectively avoided;

The reaction buffer is fully optimized, the PCR amplification efficiency is high, and the reaction sensitivity is higher;

The optimized Tag Primer is provided free of charge, and the amplification effect of miRNA is more guaranteed.

Product composition

component	**0T	**0T	**0T
miRNA SYBR Mastermix	1.0ml × 2	1.0ml × 3	1.0ml × 4
Tag Primer	*0ul	**0ul	**0ul
ddH2O	1.0ml × 2	1.0ml × 3	1.0ml × 4
U*-reward primer	*0ul × 2	*0ul × 3	*0ul × 4

Instructions:

1. Prepare the reaction system according to the table below:

Take an RNase-free centrifuge tube and prepare the following mixed solution:

2× miRNA SYBR Mastermix	*0ul
Tag Primer（*0uM）	0.4ul
Specific primer（*0uM）	0.4ul
Temlate DNA/cDNA	0.2ul
ddH2O	to *0ul

* You can design and synthesize miRNA forward primers yourself.

Note: 1. Generally speaking, a final concentration of 0.2μM primers in the reaction system can achieve a better amplification effect. When the reaction results are not ideal, the primer concentration can be adjusted within the range of 0.**1.0μM.

2. The sample volume is generally not more than 2.0ul. It is recommended to optimize and explore for the first experiment. You can take 2.0ul RT stock solution and 2, 4, 8, *6, *2, and *4 times dilutions for qPCR.

3. If the PCR results are not ideal, consider using the *-stage temperature setting for amplification. The amplification conditions are: *5℃ 5min; *5℃ *0S; *0℃ *0S; *0℃ *0S (collecting fluorescence signals).

4. Avoid strong light exposure during reagent storage and operation.

5. When preparing the reaction system, avoid excessive shaking to prevent bubbles.

Quality control

Using the synthesized U6 as a sample, using this kit, adding A, reverse transcription, taking 1/*0 cDNA product for qPCR, the CT value is less than *0.

国家:	China
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离岸价格:	（面議） 获取最新报价
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產品組 :	Molecular Biology